Gel Electrophoresis of Sunday’s PCR Product (Tues 02.28.17)
Today we ran two gels to see what we got from Sunday’s PCR. Because we had limited time Emily Chen and I can two gels. Our sample names are based on the PCR tubes.
Made 1.5% Agarose gels (3g agarose in 200ml 1x TAE)
Add SYBRsafe to gels.
I (Regina 🙂 ran a small gel with A3-A9. Emily Chen ran the larger gel with C3-C11.
We used a NEB Ready-load 100bp ladder and a Qiagen sample loading buffer. Thank-you to Riti for making the gel sheets to help with recording. (Being an idiot I forgot to log my run time. It was ~30min.)
I was able to get faint smearing bands for A3-A9. The different between these samples was the annealing temperatures. The wells for this gel were very large for a small gel. I think this might be why the bands are not very crisp; however the ladder turned out great.
Emily’s gel (C3-C11) contained no bands except for the ladder (no picture). Thus primer set 3 might be the problem.