Gel Electrophoresis

Posted on

Gel Electrophoresis of Sunday’s PCR Product (Tues 02.28.17) Today we ran two gels to see what we got from Sunday’s PCR. Because we had limited time Emily Chen and I can two gels. Our sample names are based on the PCR tubes. Made 1.5% Agarose gels (3g agarose in 200ml 1x TAE) Add SYBRsafe to […]

Salmon is back and running!

Posted on

Chelex Extraction and PCR      Sunday 02.26.2017 Hi everyone, We decided to check out protocols by doing independent runs on the weekends. Here’s my update. Salmon DNA by Chelex Extraction Measure a .6mg piece of salmon from freezer Added to 300ul of 5% Chelex and vortexed for 30sec Incubated at 95C for 6min Vortexed […]

Diving into 2017 with NerdNite Seattle

Posted on

Welcome to 2017 our fishy friends! Michal Galdzicki, Chinook Science Officer (CSO) of SoundBio, just presented at NerdNite in Seattle! In front of an audience of kindred nerds, our chinook swam upstage to talk about our hopes and dreams for the future of DIY salmon genotyping. We hope that 2017 will be a new run for […]

Researching Different Methods

Posted on

We salmonites are continuing our search into various methods of identifying our very important SNPs. (Remember our PTC Taster lab this summer?) Some of the methods we are researching include CRISPR-Cas9, Southern Blotting, and QT-PCR. Click here for a great video of Wakanene in our garage lab offering a simplified version of what the powerful […]

Summer in Seattle

Posted on

Hey everyone! We’re back to share a brief update as summer sun in Seattle is a sacred thing. Attention has been focused on projects such as travel, kite-boarding, hiking…and organizing SoundBio workshops! Here are just a few photos from our Painting With Bacteria Workshop at Amazon’s MakerSpace. It’s always a hit and we’ll definitely do it again in […]

PTC Taster (TAS2R38)

Posted on

July 5, 2016 Our group really enjoyed learning about the PTC Taster/Nontaster that took place over multiple lab sessions. This is an excellent experiment to follow in order to gain a better understanding and prepare for using restriction enzymes to genotype SNPs. The diagram below shows the Tas2R38 gene. Underlined are 3 SNPs that are associated with the taster allele. The […]

Using Restriction Enzymes to Identify SNPs

Posted on

June 28, 2016 Due to exciting future Citizen Salmon events we’ve been a bit slow on the Bioinformatics front. We are currently focused on the concept of using restriction enzymes to identify what alleles a sample may have for a specific SNP. Remember that SNPs are single nucleotide polymorphisms, and can present themselves as single base […]